Testing Protocol · ISO-Accredited Partners

How We Test Every Peptide.
Nothing Left to Chance.

Purity isn't a claim — it's a measurement. This page walks through the six-stage quality pipeline that every Skye Peptides batch passes through before it ever reaches your lab bench.

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Testing at a Glance

6+ Analytical gates per batch

100% Batches independently verified

≥99% Minimum HPLC purity standard

∞ COAs archived publicly, forever

HPLC + MS Verification

Endotoxin & Sterility Screen

Heavy-Metal Analysis (ICP-MS)

U.S. Manufacturing & Handling

Six Gates Before Release

The quality pipeline, end to end.

Every Skye Peptides batch moves through the same six-stage testing sequence — no exceptions, no shortcuts, no matter the compound or quantity.

Synthesis

Solid-phase peptide synthesis in our U.S. facility using pharmaceutical-grade reagents. LPS is removed during synthesis — never after.

HPLC Purification

Every fraction is purified via High-Performance Liquid Chromatography. Only lots at ≥99% purity advance.

Lyophilization

Freeze-dried under vacuum to preserve molecular integrity. Sealed in inert atmosphere to prevent oxidation.

Internal QC

In-house pH, quantity, and vacuum-seal verification. Any failed check triggers rejection — no exceptions.

Third-Party Analysis

Batch samples sent to independent labs for HPLC, MS, sterility, endotoxin, and heavy-metal testing.

COA Publication

The full certificate is published to our public library and shipped with the order. Searchable by batch number, forever.

The Analytical Methods

What we measure — and why it matters.

Each test tells you something specific about a batch. Together, they give you the complete picture of what's in the vial.

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HPLC Purity

High-Performance Liquid Chromatography separates your target peptide from any synthesis byproducts. The result is expressed as a percentage of total peak area — we publish it exactly as reported.

Standard: ≥99.0%

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Mass Spectrometry (MS)

Confirms the molecular weight of the peptide matches the expected sequence. If it doesn't match, the batch fails and never ships — no matter how high the HPLC purity reads.

Identity verification

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Endotoxin Testing (LAL)

Limulus Amebocyte Lysate testing measures bacterial endotoxins — critical for any research involving cell culture or in-vitro assays. Our acceptance limit is ≤5 EU/mg.

Limit: ≤5 EU/mg

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Sterility Testing

Each batch is cultured under standard conditions to detect microbial contamination. A pass means no detectable bacterial or fungal growth under tested conditions.

Pass / Fail threshold

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Heavy-Metal Analysis

ICP-MS screening for lead, arsenic, mercury, and cadmium ensures no residual metal contamination from synthesis, purification, or handling.

ICP-MS screen

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Batch Traceability

Every COA is tied to a specific batch number printed on the vial. You can verify that the certificate you're reading matches the exact lot you received.

100% traceable

Independent Verification

Tested by third-party labs you already know.

We don't self-certify. Every batch goes to one or more independent, ISO-accredited analytical labs — and we publish their reports unedited.

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Janoshik Analytical

Independent HPLC, mass spec, sterility, and endotoxin testing for research peptides. Reports are publicly verifiable via unique URLs.

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AccuMark Labs

Third-party HPLC and purity verification. Each report carries a unique verification code accessible via AccuVerify.

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Our In-House Lab

Internal QC for pH, quantity, and vacuum-seal integrity. All batches double-checked before third-party shipment.

Reading Your COA

What every certificate tells you.

A Skye Peptides Certificate of Analysis isn't marketing — it's raw data from the testing lab. Here's what each metric means.

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Purity %

The HPLC-derived purity of your target peptide. Higher is better — we require ≥99% and typically deliver 99.5%+.

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Mass (Da)

The measured molecular weight of the peptide, confirmed to match the expected theoretical mass.

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Batch Number

A unique identifier printed on your vial. Match it to your COA to verify the exact lot you received.

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Endotoxin (EU/mg)

LAL-derived bacterial endotoxin level. Lower is better; our acceptance limit is ≤5 EU/mg.

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Sterility

Pass/fail result from a microbial culture test. "Pass" means no detectable growth under tested conditions.

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Test Date

When the third-party lab completed its analysis. Most reports are issued within 30 days of synthesis.

Standards Comparison

How we compare to the industry.

Not all peptide suppliers test to the same standards. Here's a transparent look at where Skye Peptides sits.

Metric	Skye Peptides	Industry Standard	Generic Vendors
Minimum HPLC Purity	≥99%	95–98%	Often unverified
Third-Party Testing	Every batch	Every batch	Self-certified
Mass Spec Confirmation	Yes	Sometimes	Rarely
Sterility Testing	Every batch	Selected batches	Not typically
Endotoxin (LAL)	≤5 EU/mg	Varies widely	Unverified
Heavy-Metal Analysis	ICP-MS	Occasional	Not offered
Public COA Library	Free & permanent	Available on request	Not published
Batch Traceability	Vial-printed ID	Sometimes	Often missing

Our testing standards.

The numbers we hold every single batch to — without exception.

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≥99% Purity HPLC-verified minimum. Failure = rejection.

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≤5 EU/mg Endotoxin Typically delivered below 0.2 EU/mg.

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Sterility: Pass No detectable microbial growth.

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Heavy Metals: None ICP-MS confirmed below detection limits.

Frequently Asked

Technical questions researchers ask.

Can I verify my COA directly with the testing lab?

Yes. Every Janoshik report on our Test Reports page includes a unique verification URL — click it to open the report directly on Janoshik's servers. AccuMark reports use the AccuVerify system with unique codes. You're never forced to trust our copy of the data.

What happens if a batch fails a test?

Batches that fail any of our six gates are destroyed, never sold. We do not "rework," "re-label," or discount failed batches. If it doesn't pass our standards, it doesn't reach you.

Why does purity vary between batches (e.g., 99.5% vs. 99.9%)?

Every synthesis has natural variability. Small differences in purity between batches — even within the same compound — are normal and reflect real analytical measurements. All Skye batches exceed our ≥99% minimum. If you need a specific batch purity, check the COA before ordering.

Do you test for heavy metals on every batch?

Yes. Heavy-metal testing (lead, arsenic, mercury, cadmium) is part of our standard panel, not an add-on. The results are published on the same COA as purity and sterility.

Can I request additional testing on a specific batch?

For larger institutional orders, yes — we can commission additional tests through our partner labs on request. Email support@blueskyepeptides.com with your requirements and batch number.

Are your labs ISO-accredited?

Yes — our partner labs (Janoshik, AccuMark) operate under ISO-accredited quality systems for analytical chemistry. Each published report includes the lab's own certification and verification credentials.

Where can I download COAs for previous orders?

All current and historic batch COAs are published on our Test Reports page. Search by peptide name or batch number. If you can't find a specific batch, email us and we'll send it within 24 hours.

See our standards in action.

Every batch of every peptide we sell has a published, independently-verified certificate. Browse the full library — searchable by name, batch, or CAS.

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Research-Use Only

All peptides sold by Skye Peptides are intended exclusively for in-vitro laboratory research. They are not drugs, foods, cosmetics, or any article intended for human or animal consumption.

Test results published on this page reflect analytical data for research identification and quality assurance purposes only. They are not intended as medical claims or endorsement of any non-research use.